Flash hex file with bootloader or without? Hardware, firmware and software help

According to a few guides I’ve read, ARC_SUPPORT isn’t something that 3D printers normally use, so I disabled that by commenting out #define ARC_SUPPORT in Configuration_adv.h. Again, many of the guides online suggest using Arduino IDE. This is probably convenienten savoir +Flash hex file with bootloader or without? Hardware, firmware and software help